Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden.
Material type: TextSeries: Microscopy handbooks ; 37.Publication details: Oxford, UK : BIOS Scientific Publishers ; New York : Springer ; c1997.Edition: 2nd edDescription: xiv, 141 p. : ill. (some col.) ; 24 cmISBN:- 0387915133 (New York)
- 571.964 21 POL-I 1997 501430
Item type | Current library | Collection | Call number | Status | Date due | Barcode | Item holds | |
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Reference | University College of Medicine Library Shelf No. 33, LBS | Not for loan and check out is restricted | 571.964 POL-I 1997 501430 (Browse shelf(Opens below)) | Not For Loan (Restricted Access) | 501430 |
"In association with the Royal Microscopical Society."
Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index.