Introduction to immunocytochemistry /
Polak, Julia M.
Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden. - 2nd ed. - Oxford, UK : New York : BIOS Scientific Publishers ; Springer ; c1997. - xiv, 141 p. : ill. (some col.) ; 24 cm. - Royal Microscopical Society microscopy handbooks ; 37 . - Microscopy handbooks ; 37. .
"In association with the Royal Microscopical Society."
Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index.
0387915133 (New York)
Immunohistochemistry
571.964 / POL-I 1997 501430
Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden. - 2nd ed. - Oxford, UK : New York : BIOS Scientific Publishers ; Springer ; c1997. - xiv, 141 p. : ill. (some col.) ; 24 cm. - Royal Microscopical Society microscopy handbooks ; 37 . - Microscopy handbooks ; 37. .
"In association with the Royal Microscopical Society."
Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index.
0387915133 (New York)
Immunohistochemistry
571.964 / POL-I 1997 501430