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Introduction to immunocytochemistry / J.M. Polak, S. Van Noorden.

By: Contributor(s): Material type: TextTextSeries: Microscopy handbooks ; 37.Publication details: Oxford, UK : BIOS Scientific Publishers ; New York : Springer ; c1997.Edition: 2nd edDescription: xiv, 141 p. : ill. (some col.) ; 24 cmISBN:
  • 0387915133 (New York)
Subject(s): DDC classification:
  • 571.964 21 POL-I 1997 501430
Contents:
Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index.
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Holdings
Item type Current library Collection Call number Status Date due Barcode Item holds
Reference Reference University College of Medicine Library Shelf No. 33, LBS Not for loan and check out is restricted 571.964 POL-I 1997 501430 (Browse shelf(Opens below)) Not For Loan (Restricted Access) 501430
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"In association with the Royal Microscopical Society."

Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index.

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