TY - BOOK AU - Polak,Julia M. AU - Van Noorden,Susan ED - Royal Microscopical Society (Great Britain) TI - Introduction to immunocytochemistry T2 - Royal Microscopical Society microscopy handbooks SN - 0387915133 (New York) U1 - 571.964 21 PY - 1997/// CY - Oxford, UK, New York PB - BIOS Scientific Publishers, Springer KW - Immunohistochemistry N1 - "In association with the Royal Microscopical Society."; Introduction -- Production of antibodies -- Requirements for immunocytochemistry -- Colour plates -- Methods -- Specificity problems and essential controls -- Enhancement of standard methods -- Multiple immunostaining -- Post-embedding immunocytochemistry for the transmission electron microscope -- In vitro methods for testing antigen-antibody reactions -- Applications of immunocytochemistry -- Microscopy -- Appendix: technical notes -- Buffers -- Antibody diluent and storage of antibodies -- Adherence of preparations to slides -- Blocking endogenous peroxidase reaction -- Enzyme pre-treatment -- Heat-mediated antigen retrieval using a microwave oven -- Enzyme development methods -- Intensifying the peroxidase/DAB reaction product -- Immunostaining methods -- Immunogold staining with silver enhancement -- Double immunoenzymatic staining -- Post-embedding electron microscopical -- Absorption specificity control (liquid phase) -- References -- Index ER -